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KMID : 1094720090140010105
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Biotechnology and Bioprocess Engineering
2009 Volume.14 No. 1 p.105 ~ p.111
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Effects of Oxygen Volumetric Mass Transfer Coefficient and pH on Lipase Production by Staphylococcus warneri EX17
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Giandra Volpato
Rafael C. Rodrigues
Julio Xandro Heck
Marco Antonio Zachia Ayub
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Abstract
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The principal objectives of this study were to evaluate the kinetics of lipase production by Staphylococcus warneri EX17 under different oxygen volumetric mass transfer coefficients (kLa) and pH conditions in submerged bioreactors, using glycerol (a biodiesel by-product) as a carbon source. Cultivations were conducted at different kLa (26, 38, 50, and 83 h-1) and pH values (6.0, 7.0, and 8.0). The optimal kLa and pH were 38 h-1 and 7.0, respectively. Under these conditions, the maximal cell production obtained was 8.0 g/L, and the volumetric and specific lipase production reached high levels of activity, approximately 800 U/L and 150 U/g cell, respectively, after 12 h of cultivation. This result was approximately five times higher than that obtained in the shake flask cultures. The relationship between cell growth and lipase production was found to be associated with growth by the Luedeking-Piret model.
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KEYWORD
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lipase, Staphylococcus warneri, bioreactor, oxygen volumetric mass transfer coefficient (kLa)
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